A new approach for the preparation of a biochip on porous silicon and the application of the biochip for detection of small molecule-protein interactions with desorption/ionization on porous silicon (DIOS) was demonstrated. The galvanostatically etched porous silicon substrates were chemically modified firstly to yield carboxylic acid terminated surfaces, and then the protein was covalently attached to the surface through amide bonding. By applying a solution of candidate chemicals to the surface and a subsequent wash step, the masses of captured compounds could be analyzed by DIOS. DIOS has advantages of being a direct detection tool compared to the classic florescence or chemiluminescence methods, because the process of labeling molecules employed in the florescence or chemiluminescence methods can sometimes alert the properties of the labeled molecule. The recognition between proteins and their binding partners is efficient and selective. A good tolerance to disturbance and high enrichment factor of the biochip to the analytes was observed. As an on-chip-based approach, the demonstrated method has a potential to perform in a high-throughput format. Copyright (c) 2007 John Wiley & Sons, Ltd.
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机译:展示了一种在多孔硅上制备生物芯片的新方法以及该生物芯片在检测小分子-蛋白质相互作用以及多孔硅上的解吸/电离(DIOS)方面的应用。首先对经过恒电流刻蚀的多孔硅基板进行化学修饰,以产生末端为羧酸的表面,然后通过酰胺键将蛋白质共价连接至表面。通过将候选化学物质的溶液施加到表面并随后进行清洗步骤,可以通过DIOS分析捕获的化合物的质量。与经典的荧光或化学发光方法相比,DIOS具有作为直接检测工具的优势,因为在荧光或化学发光方法中使用的标记分子的过程有时会提示被标记分子的特性。蛋白质及其结合伴侣之间的识别是有效和选择性的。观察到生物芯片对干扰物的良好耐受性和高富集因子。作为基于芯片的方法,已证明的方法具有以高通量格式执行的潜力。版权所有(c)2007 John Wiley&Sons,Ltd.
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